Intermediate R/Bioconductor for High-Throughput Sequence Analysis introduces users with some R experience to common Bioconductor work flows for sequence analysis. The course involves a combination of presentations and hands-on exercises. Our starting point is BAM files created by aligning short reads to a reference genome. Topics include exploratory analysis (GenomicRanges, Rsamtools); assessing differential expression of known genes (DESeq); detection, calling, and manipulation of variants (VariantTools, VariantAnnotation). We learn how to integrate results with curated gene and genomic annotations (GenomicFeatures), and to visualize results (GViz, ggbio).……【阅读全文】

前文《生成特定碱基比例的随机序列》介绍了如何用Perl来生成特定碱基比例的随机序列。……【阅读全文】

Bioconductor官网主页
Bioconductor Workflows（简单的实例）
Bioconductor FAQ（常见问题解答）
Bioconductor Courses & Conferences（无价的资料）……【阅读全文】