RNA-seq is a recent and immensely popular technology for cataloging and comparing gene expression. Two papers from the international RGASP consortium report on large-scale competitions to identify the best algorithms for RNA-seq analysis, with surprising variability in the results.……【阅读全文】
Robasky, K., Lewis, N. E. & Church, G. M. The role of replicates for error mitigation in next-generation sequencing. Nat. Rev. Genet. 1–7 (2013). doi:10.1038/nrg3655……【阅读全文】
Intermediate R/Bioconductor for High-Throughput Sequence Analysis introduces users with some R experience to common Bioconductor work flows for sequence analysis. The course involves a combination of presentations and hands-on exercises. Our starting point is BAM files created by aligning short reads to a reference genome. Topics include exploratory analysis (GenomicRanges, Rsamtools); assessing differential expression of known genes (DESeq); detection, calling, and manipulation of variants (VariantTools, VariantAnnotation). We learn how to integrate results with curated gene and genomic annotations (GenomicFeatures), and to visualize results (GViz, ggbio).……【阅读全文】
日前，两项刊登在《自然-方法学》（Nature Methods）上的研究对比了用于分析从单个细胞所有 RNA 获得的测序数据的不同计算工具。……【阅读全文】
12月21日出版的美国《科学》杂志认为，2013年有6大科学领域值得关注：单细胞测序、“普朗克”探测微波背景辐射、人类连接组计划、探索南极冰下世界、癌症免疫疗法和基础植物研究。……【阅读全文】
美国《时代》杂志评选的各领域年度“十大”排名已于近日陆续出炉，医学领域“十大”突破也深入人心。涵盖了生命基础研究、艾滋病与癌症治疗突破、干细胞与再生医学、青少年健康等多方面公众关心的热点。……【阅读全文】
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